Southern blot is a molecular genetic method for DNA determination, and it works as follows:
First separates its restriction fragments by gel electrophoresis. DNA on the gel is denatured and transferred to a positively charged membrane. DNA migrates to the membrane, because it is itself negatively charged, under non-acidic conditions. Then fastened it to the membrane using UV irradiation or heating.
DNA is exposed as a hybridiseringsprobe containing methylated DNA sequences similar to restriction fragment, to test for. These sequences are labeled for example with a fluorescent substance or that it has incorporated 32P, which is radioactive in the DNA backbone. If the membrane containing restriction fragment, exploring for, they will hybridize with probesekvenserne.
